The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas

doi:10.3389/fimmu.2018.00284

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	The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas
	Liu, Zhaoqun 1,2,3; Wang, Lingling 1,2; Lv, Zhao 3,4; Zhou, Zhi 5; Wang, Weilin 1; Li, Meijia 3,4; Yi, Qilin 1; Qiu, Limei 3; Song, Linsheng 1,2
	2018-02-26
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	9
摘要	It is becoming increasingly clear that neurotransmitters impose direct influence on regulation of the immune process. Recently, a simple but sophisticated neuroendocrine-immune (NEI) system was identified in oyster, which modulated neural immune response via a "nervous-hemocyte"-mediated neuroendocrine immunomodulatory axis (NIA)-like pathway. In the present study, the de novo synthesis of neurotransmitters and their immunomodulation in the hemocytes of oyster Crassostrea gigas were investigated to understand the autocrine/paracrine pathway independent of the nervous system. After hemocytes were exposed to lipopolysaccharide (LPS) stimulation, acetylcholine (ACh), and norepinephrine (NE) in the cell supernatants, both increased to a significantly higher level (2.71-and 2.40-fold, p < 0.05) comparing with that in the control group. The mRNA expression levels and protein activities of choline O-acetyltransferase and dopamine beta-hydroxylase in hemocytes which were involved in the synthesis of ACh and NE were significantly elevated at 1 h after LPS stimulation, while the activities of acetylcholinesterase and monoamine oxidase, two enzymes essential in the metabolic inactivation of ACh and NE, were inhibited. These results demonstrated the existence of the sophisticated intracellular machinery for the generation, release and inactivation of ACh and NE in oyster hemocytes. Moreover, the hemocyte-derived neurotransmitters could in turn regulate the mRNA expressions of tumor necrosis factor (TNF) genes, the activities of superoxide dismutase, catalase and lysosome, and hemocyte phagocytosis. The phagocytic activities of hemocytes, the mRNA expressions of TNF and the activities of key immune-related enzymes were significantly changed after the block of ACh and NE receptors with different kinds of antagonists, suggesting that autocrine/paracrine self-regulation was mediated by transmembrane receptors on hemocyte. The present study proved that oyster hemocyte could de novo synthesize and release cholinergic and adrenergic neurotransmitters, and the hemocyte-derived ACh/NE could then execute a negative regulation on hemocyte phagocytosis and synthesis of immune effectors with similar autocrine/paracrine signaling pathway identified in vertebrate macrophages. Findings in the present study demonstrated that the immune and neuroendocrine system evolved from a common origin and enriched our knowledge on the evolution of NEI system.
文章类型	Article
关键词	Crassostrea Gigas Autocrine/paracrine Hemocyte Neurotransmitter Membrane Receptor Immune Regulation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fimmu.2018.00284
关键词[WOS]	SCALLOP CHLAMYS-FARRERI ; DOPAMINE-BETA-HYDROXYLASE ; MUSCARINIC ACETYLCHOLINE-RECEPTOR ; NERVOUS-SYSTEM ; T-CELLS ; BACTERIAL CHALLENGE ; IMMUNE-RESPONSE ; MESSENGER-RNA ; TNF-ALPHA ; EXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Immunology
项目资助者	National Science Foundation of China, AoShan Talents Cultivation Program(U1706204) ; Qingdao National Laboratory for Marine Science and Technology(2017ASTCP-OS13) ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research ; Dalian High Level Talent Innovation Support Program(2015R020) ; Research Foundation for Talented Scholars in Dalian Ocean University ; Distinguished Professor of Liaoning
WOS类目	Immunology
WOS记录号	WOS:000426069400001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10620
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Wang, Lingling; Song, Linsheng
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhaoqun,Wang, Lingling,Lv, Zhao,et al. The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2018,9.
APA	Liu, Zhaoqun.,Wang, Lingling.,Lv, Zhao.,Zhou, Zhi.,Wang, Weilin.,...&Song, Linsheng.(2018).The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,9.
MLA	Liu, Zhaoqun,et al."The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 9(2018).