Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps

doi:10.1016/j.fsi.2017.11.004

QIBEBT-IR

	Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps
	Wen, Li-lian 1,2,3; Zhao, Ming-li 1,2,3; Chi, Heng 1,2; Sun, Li 1,2
	2018
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	72 页码:470-476
摘要	Neutrophil extracellular traps (NETs) are a form of extracellular antimicrobial structure of neutrophils observed in higher and lower vertebrates, the latter including the teleost fish tongue sole Cynoglossus semilaevis. However, the antimicrobial mechanism of fish NETs is unknown. In the present study, we examined the potential contribution of histones and elastases to the antibacterial effect of tongue sole NETs. For this purpose, two histones (CsH2B and CsH4) and two elastases (CsElal and CsEla2) of tongue sole were investigated. The histones and elastases possess the conserved domain structures characteristic of that of histones H2B/H4 and trypsin-like serine protease, respectively. Recombinant CsH2B, CsH4, CsElal, and CsEla2 bound a wide range of Gram-negative and Gram-positive bacteria, and some of the bound bacteria were inhibited in growth by the bound histones/elastases. CsH2B, CsH4, CsElal, and CsEla2 were all localized in NETs induced by various stimuli including bacterial pathogen. Treatment of NETs with antibodies targeting CsH2B, CsH4, CsElal, and CsEla2 significantly reduced the antimicrobial effect of NETs. These results indicate that histones and chymotrypsin-like elastases are fundamental components of teleost NETs that play important roles in the antimicrobial activity of NETs.
文章类型	Article
关键词	Neutrophil Extracellular Traps Histone Elastase Cynoglossus Semilaevis Antimicrobial
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.fsi.2017.11.004
关键词[WOS]	CYNOGLOSSUS-SEMILAEVIS ; BACTERIAL-INFECTION ; SCOPHTHALMUS-MAXIMUS ; HUMAN-GRANULOCYTES ; HOST-DEFENSE ; PROTEINS ; LEUKOCYTE ; GRANULES ; KIDNEY ; DEGRADATION
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
项目资助者	National Natural Science Foundation of China(41576150 ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Taishan Scholar Program of Shandong Province ; 31330081)
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000423644600055
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10566
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wen, Li-lian,Zhao, Ming-li,Chi, Heng,et al. Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:470-476.
APA	Wen, Li-lian,Zhao, Ming-li,Chi, Heng,&Sun, Li.(2018).Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps.FISH & SHELLFISH IMMUNOLOGY,72,470-476.
MLA	Wen, Li-lian,et al."Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps".FISH & SHELLFISH IMMUNOLOGY 72(2018):470-476.