Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection

doi:10.1016/j.dci.2017.10.008

QIBEBT-IR

	Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection
	Li, Mo-Fei 1,2; Sun, Li 1,2
	2018-02-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	79 页码:142-149
摘要	In mammals, membrane-associated complement regulatory proteins (MCRP) can protect host cells from the damaging of the activated complement. In teleost, few studies on the function of MCRP have been documented. In the present report, we identified a MCRP (named CsMCRP) from the teleost fish tongue sole Cynoglossus semilaevis and examined its immune function. CsMCRP shares moderate sequence identities with fish DAF-like molecules. CsMCRP was predicted to be a transmembrane protein with three short consensus repeats located in the extracellular region. CsMCRP expression occurred in nine different tissues, especially blood, and in peripheral blood leukocytes (PBL). Recombinant CsMCRP inhibited complement activation and interacted with bacterial pathogen, the latter in a highly selective manner. Antibody blocking the CsMCRP on PBL significantly inhibited bacterial infection of PBL. These results indicate that teleost CsMCRP is both a regulator of complement activation and a cellular receptor involved in bacterial invasion. (C) 2017 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Complement Regulatory Protein Complement Activation Cynoglossus Semilaevis Bacterial Infection
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.dci.2017.10.008
关键词[WOS]	DECAY-ACCELERATING FACTOR ; SOLE CYNOGLOSSUS-SEMILAEVIS ; FACTOR DAF ; ALTERNATIVE PATHWAY ; COFACTOR PROTEIN ; FACTOR CD55 ; IN-VIVO ; CELLS ; EXPRESSION ; SYSTEM
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Zoology
项目资助者	National Natural Science Foundation of China(31330081) ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Taishan Scholar Program of Shandong Province
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000422811500016
出版者	ELSEVIER SCI LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10548
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Mo-Fei,Sun, Li. Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,79:142-149.
APA	Li, Mo-Fei,&Sun, Li.(2018).Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,79,142-149.
MLA	Li, Mo-Fei,et al."Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 79(2018):142-149.