BPN, a marine-derived PTP1B inhibitor, activates insulin signaling and improves insulin resistance in C2C12 myotubes

doi:10.1016/j.ijbiomac.2017.08.042

QIBEBT-IR

	BPN, a marine-derived PTP1B inhibitor, activates insulin signaling and improves insulin resistance in C2C12 myotubes
	Xu, Qi 1,2,3; Luo, Jiao 1,2,3; Wu, Ning 1,3; Zhang, Renshuai 1,3; Shi, Dayong 1,2,3
	2018
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	106 页码:379-386
摘要	Insulin resistance is a key feature of type 2 diabetes mellitus (T2DM) and is characterized by defects in insulin signaling. Protein tyrosine phosphatase 1B (PTP1B) is a major negative regulator of insulin signaling cascade and has attracted intensive investigation in recent T2DM therapy study. BPN, a marine derived bromophenol compound, was isolated from the red alga Rhodomela confervoides. This study investigated the effects of BPN on the insulin signaling pathway in insulin-resistant C2C12 myotubes by inhibiting PTP1B. Molecular docking study and analysis of small-molecule interaction with PTP1B all showed BPN inhibited PTP1B activity via binding to the catalytic site through hydrogen bonds. We then found that BPN permeated into C2C12 myotubes, on the one hand, activated insulin signaling in an insulin independent manner in C2C12 cells; on the other hand, ameliorated palmitate-induced insulin resistance through augmenting insulin sensitivity. Moreover, our studies also showed that FTP1B inhibition by BPN increased glucose uptake in normal and insulin-resistant C2C12 myotubes through glucose transporter 4 (GLUT4) translocation. Taken together, BPN activates insulin signaling and alleviates insulin resistance and represents a potential candidate for further development as an antidiabetic agent. (C) 2017 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Bpn Protein Tyrosine Phosphatase 1b Insulin Signaling Insulin Resistance C2c12 Myotubes
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.ijbiomac.2017.08.042
关键词[WOS]	TYROSINE-PHOSPHATASE 1B ; GLUCOSE TRANSPORTERS ; OBESITY ; BROMOPHENOLS ; DERIVATIVES ; EXPRESSION ; DESIGN ; CANCER ; CELLS ; RATS
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
项目资助者	National Natural Science Foundation of China(41506169) ; Key research and development project of Shandong province(2015GSF115029 ; Key Research Program of Frontier Sciences, CAS(QYZDB-SSW-DQC014) ; Project of Discovery, Evaluation and Transformation of Active Natural Compounds ; Strategic Biological Resources Service Network Programme of Chinese Academy of Sciences(ZSTH-026) ; NSFC-shandong Joint Fund for Marine Science Research Centers(U1606403) ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02) ; National Program for Support of Top-notch Young Professionals ; Taishan scholar Youth Project of Shandong province ; Aoshan Talents Program - Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; 2016ZDJS07A13 ; 2016GSF115002 ; 2016GSF201193)
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000417661600043
出版者	ELSEVIER SCIENCE BV
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10520
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Shi, Dayong
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Xu, Qi,Luo, Jiao,Wu, Ning,et al. BPN, a marine-derived PTP1B inhibitor, activates insulin signaling and improves insulin resistance in C2C12 myotubes[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,106:379-386.
APA	Xu, Qi,Luo, Jiao,Wu, Ning,Zhang, Renshuai,&Shi, Dayong.(2018).BPN, a marine-derived PTP1B inhibitor, activates insulin signaling and improves insulin resistance in C2C12 myotubes.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,106,379-386.
MLA	Xu, Qi,et al."BPN, a marine-derived PTP1B inhibitor, activates insulin signaling and improves insulin resistance in C2C12 myotubes".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 106(2018):379-386.