QIBEBT-IR  > 生物基材料组群
High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli
Liu, Hui1; Cheng, Tao1; Zou, Huibin1,2; Zhang, Haibo1; Xu, Xin1; Sun, Chao1; Aboulnaga, Elhussiny1,3; Cheng, Zhongkai4; Zhao, Guang1; Xian, Mo1
2017-11-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume62Pages:1-9
AbstractIsoprenoids are important fine chemicals as material monomers, advanced fuels and pharmaceuticals. A variety of natural isoprenoids can be synthesized by engineered microbial strains. This work established a process by dividing the current isoprenoid pathway into the upstream fermentation process, from sugar to mevalonate (MVA), and the downstream process, from MVA to the target isoprenoids. The results showed that significant differences existed in the process conditions between the upstream and downstream fermentations. After individually optimizing the process conditions, the upstream MVA production (84.0 g/L, 34.0% and 1.8 g/ L/h) and downstream isoprene production (11.0 g/L and 0.23 g/L/h) were greatly improved in this two-step process. Flask fermentation experiments also confirmed that two-step route can significantly improve the sabinene titer to 150 mg/L (6.5-fold of the sabinene titer in an earlier flask study of our lab). Therefore, the two-step route proposed in this study may have potential benefits towards the current isoprenoids production directly from glucose. The high titer and yield of MVA indicate that MVA has great potential to be more broadly utilized as starting precursor in synthetic biology.
SubtypeArticle
KeywordMevalonate Isoprene Sabinene Betaine Biosynthesis
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2017.07.021
WOS KeywordBACILLUS-SUBTILIS ; ISOPENTENOL PRODUCTION ; SYNTHETIC BIOLOGY ; PATHWAY ; STRAIN ; IDENTIFICATION ; OPTIMIZATION ; SYNTHASES ; MICROBES ; GENES
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
Funding OrganizationShandong Province Natural Science Foundation(ZR2015BM011) ; Technology Development Project of Shandong Province(2016GSF121013) ; National Natural Science Foundation(21106170 ; 100 Talent Project of CAS ; 21376129 ; 21572242 ; 31670493)
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000416394500001
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/10484
Collection生物基材料组群
Corresponding AuthorZou, Huibin; Zhao, Guang; Xian, Mo
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
2.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
3.Mansoura Univ, Fac Agr, Mansoura 35516, Egypt
4.Jilin Univ, Sch Life Sci, Changchun, Jilin, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hui,Cheng, Tao,Zou, Huibin,et al. High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli[J]. PROCESS BIOCHEMISTRY,2017,62:1-9.
APA Liu, Hui.,Cheng, Tao.,Zou, Huibin.,Zhang, Haibo.,Xu, Xin.,...&Xian, Mo.(2017).High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.PROCESS BIOCHEMISTRY,62,1-9.
MLA Liu, Hui,et al."High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli".PROCESS BIOCHEMISTRY 62(2017):1-9.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Hui]'s Articles
[Cheng, Tao]'s Articles
[Zou, Huibin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Hui]'s Articles
[Cheng, Tao]'s Articles
[Zou, Huibin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Hui]'s Articles
[Cheng, Tao]'s Articles
[Zou, Huibin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.