High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli

doi:10.1016/j.procbio.2017.07.021

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	High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli
	Liu, Hui1 ; Cheng, Tao1 ; Zou, Huibin 1,2; Zhang, Haibo1 ; Xu, Xin1 ; Sun, Chao1 ; Aboulnaga, Elhussiny 1,3; Cheng, Zhongkai 4; Zhao, Guang 1; Xian, Mo1
	2017-11-01
发表期刊	PROCESS BIOCHEMISTRY
ISSN	1359-5113
卷号	62 页码:1-9
摘要	Isoprenoids are important fine chemicals as material monomers, advanced fuels and pharmaceuticals. A variety of natural isoprenoids can be synthesized by engineered microbial strains. This work established a process by dividing the current isoprenoid pathway into the upstream fermentation process, from sugar to mevalonate (MVA), and the downstream process, from MVA to the target isoprenoids. The results showed that significant differences existed in the process conditions between the upstream and downstream fermentations. After individually optimizing the process conditions, the upstream MVA production (84.0 g/L, 34.0% and 1.8 g/ L/h) and downstream isoprene production (11.0 g/L and 0.23 g/L/h) were greatly improved in this two-step process. Flask fermentation experiments also confirmed that two-step route can significantly improve the sabinene titer to 150 mg/L (6.5-fold of the sabinene titer in an earlier flask study of our lab). Therefore, the two-step route proposed in this study may have potential benefits towards the current isoprenoids production directly from glucose. The high titer and yield of MVA indicate that MVA has great potential to be more broadly utilized as starting precursor in synthetic biology.
文章类型	Article
关键词	Mevalonate Isoprene Sabinene Betaine Biosynthesis
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1016/j.procbio.2017.07.021
关键词[WOS]	BACILLUS-SUBTILIS ; ISOPENTENOL PRODUCTION ; SYNTHETIC BIOLOGY ; PATHWAY ; STRAIN ; IDENTIFICATION ; OPTIMIZATION ; SYNTHASES ; MICROBES ; GENES
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
项目资助者	Shandong Province Natural Science Foundation(ZR2015BM011) ; Technology Development Project of Shandong Province(2016GSF121013) ; National Natural Science Foundation(21106170 ; 100 Talent Project of CAS ; 21376129 ; 21572242 ; 31670493)
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号	WOS:000416394500001
出版者	ELSEVIER SCI LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10484
专题	生物基材料组群
通讯作者	Zou, Huibin; Zhao, Guang; Xian, Mo
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China 3.Mansoura Univ, Fac Agr, Mansoura 35516, Egypt 4.Jilin Univ, Sch Life Sci, Changchun, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hui,Cheng, Tao,Zou, Huibin,et al. High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli[J]. PROCESS BIOCHEMISTRY,2017,62:1-9.
APA	Liu, Hui.,Cheng, Tao.,Zou, Huibin.,Zhang, Haibo.,Xu, Xin.,...&Xian, Mo.(2017).High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.PROCESS BIOCHEMISTRY,62,1-9.
MLA	Liu, Hui,et al."High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli".PROCESS BIOCHEMISTRY 62(2017):1-9.