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DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum
Jiang, Shuai1; Wang, Lingling2; Huang, Mengmeng1; Jia, Zhihao1; Weinert, Tobias3; Warkentin, Eberhard4; Liu, Conghui1; Song, Xiaorui1; Zhang, Haixia1; Witt, Jennifer4; Qiu, Limei1; Peng, Guohong1,4; Song, Linsheng2
2017-11-29
发表期刊FRONTIERS IN IMMUNOLOGY
ISSN1664-3224
卷号8
摘要DM9 domain was first identified in Drosophila melanogaster, and it was subsequently found to integrate with or without other protein domains across a wide range of invertebrates and vertebrates. In the present study, a member of DM9 domain containing protein (DM9CP) family from marine invertebrate Crassostrea gigas (designated CgDM9CP-1), which was only composed of two DM9 domains, was taken as a protein model to study the biological functions of DM9 domain and its molecular determinants. CgDM9CP-1 was found to exhibit high binding specificity and avidity toward D-mannose residue. It served as a pattern recognition receptor (PRR) with a broad range of recognition spectrum to various pathogen-associated molecular patterns, including lipopolysaccharide, peptidylglycan, mannan, and beta-1, 3-glucan in a D-mannose-dependent manner, as well as bacteria and fungi. In order to reveal the molecular mechanism underlying its pattern recognition activity, the crystal structures of wild-type and loss-of-function mutants were solved, and Asp22 and Lys43 were found to be the critical residues for ligand recognition. Moreover, CgDM9CP-1 protein was found to mainly distribute on the surface of C. gigas hemocytes, and it could be translocated into cytoplasm and colocalized with the engulfed microbes during hemocyte phagocytosis. The present result clearly indicated that CgDM9CP-1 was a PRR, and it provided an important clue for the better understanding of DM9CP function.
文章类型Article
关键词Innate Immunity Pattern Recognition Receptor Dm9 Domain Crystal Structure Mannose Binding Phagocytosis
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.3389/fimmu.2017.01607
关键词[WOS]OYSTER CRASSOSTREA-GIGAS ; NATTERERI FISH VENOM ; THALASSOPHRYNE-NATTERERI ; DROSOPHILA-MELANOGASTER ; SIGNALING PATHWAY ; GENE-EXPRESSION ; INNATE IMMUNITY ; DC-SIGN ; LECTIN ; INFECTION
收录类别SCI
语种英语
WOS研究方向Immunology
项目资助者Natural Science Foundation of China(31530069 ; High Technology Project (863 Program) from the Chinese Ministry of Science and Technology(2014AA093501) ; Dalian High Level Talent Innovation Support Program(2015R020) ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Earmarked Fund for Modern Agro-industry Technology Research System(CARS-49) ; Max-Planck-Gesellschaft ; 41406170)
WOS类目Immunology
WOS记录号WOS:000416404600001
出版者FRONTIERS MEDIA SA
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/10474
专题中国科学院青岛生物能源与过程研究所
通讯作者Peng, Guohong; Song, Linsheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol Dis Control, Dalian, Peoples R China
3.Paul Scherrer Inst, Lab Biomol Res, Villigen, Switzerland
4.Max Planck Inst Biophys, Dept Mol Membrane Biol, Frankfurt, Germany
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GB/T 7714
Jiang, Shuai,Wang, Lingling,Huang, Mengmeng,et al. DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum[J]. FRONTIERS IN IMMUNOLOGY,2017,8.
APA Jiang, Shuai.,Wang, Lingling.,Huang, Mengmeng.,Jia, Zhihao.,Weinert, Tobias.,...&Song, Linsheng.(2017).DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum.FRONTIERS IN IMMUNOLOGY,8.
MLA Jiang, Shuai,et al."DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum".FRONTIERS IN IMMUNOLOGY 8(2017).
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