A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli

doi:10.1080/21655979.2017.1286432

QIBEBT-IR > 生物基材料组群

	A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli
	Lacmata, Stephen Tamekou 1,2; Yao, Lan 1; Xian, Mo 1; Liu, Hui 1; Kuiate, Jules-Roger 2; Liu, Huizhou 1; Feng, Xinjun1 ; Zhao, Guang 1
	2017
发表期刊	BIOENGINEERED
ISSN	2165-5979
卷号	8 期号:5 页码:594-599
摘要	The release of intracellular products, especially polyhydroxyalkanoates, is still a great challenge in industry. To solve this bottleneck, a novel autolysis system strictly controlled with magnesium was constructed and applied to poly(3-hydroxypropionate) production in engineered Escherichia coli. The autolysis system was constructed by inserting the 5untranslated region (5UTR) behind promoter PmgtA with lysis genes (S, R, and Rz, from E. coli) overexpressed. The autolysis system functioned well (lysis efficiency of more than 90%) in the P3HP producer with double plasmids containing lysis genes and P3HP biosynthesis genes, whereas the P3HP production was reduced due to plasmid losses. After the autolysis genes and P3HP biosynthesis genes were integrated into one plasmid, the P3HP content of 72.7% (2.4times of the control) and the plasmid stability of 79.8 +/- 3.1% were achieved in strain Q2646 with promoter PmgtA-UTR. However, the strain Q2647 with promoter PmgtA could not accumulate P3HP because of rapid cell lysis. The novel autolysis system activated in Mg2+-depleted conditions proves to be feasible for polyhydroxyalkanoates production, which may have great application potential for other intracellular products.
文章类型	Article
关键词	Autolysis System Industrial Applications Magnesium P3hp Promoter Untranslated Regions
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1080/21655979.2017.1286432
关键词[WOS]	POLY(3-HYDROXYPROPIONATE) ; MG2+ ; BIOSYNTHESIS ; GLYCEROL ; RNA
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	100-Talent Project of CAS ; Key Program of CAS(ZDRW-ZS-2016-3M ; National Natural Science Foundation of China(31670089 ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; Shandong Province Post-doctoral Innovation Foundation(201603076) ; KGZD-EW-606-1-3) ; 31670493)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000413964300017
出版者	TAYLOR & FRANCIS INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10464
专题	生物基材料组群
通讯作者	Feng, Xinjun
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Dschang, Dept Biochem, Lab Microbiol & Antimicrobials Subst, Fac Sci, Dschang, Cameroon
推荐引用方式 GB/T 7714	Lacmata, Stephen Tamekou,Yao, Lan,Xian, Mo,et al. A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli[J]. BIOENGINEERED,2017,8(5):594-599.
APA	Lacmata, Stephen Tamekou.,Yao, Lan.,Xian, Mo.,Liu, Hui.,Kuiate, Jules-Roger.,...&Zhao, Guang.(2017).A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.BIOENGINEERED,8(5),594-599.
MLA	Lacmata, Stephen Tamekou,et al."A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli".BIOENGINEERED 8.5(2017):594-599.