Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappa B/COX-2 activation and apoptosis in HaCaT cells

doi:10.1016/j.jphotobiol.2009.04.010

QIBEBT-IR

	Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappa B/COX-2 activation and apoptosis in HaCaT cells
	Liu, XiaoJin 1; Shi, ShaoTing 2; Ye, JunLi 1; Liu, LeQian 3; Sun, Mi 4; Wang, ChunBo 1
	2009-08-03
发表期刊	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
ISSN	1011-1344
卷号	96 期号:2 页码:109-116
摘要	Polypeptide from Chlamys farreri (PCF) is a novel marine polypeptide compound isolated from gonochoric Chinese scallop Chlamys farreri, this study we further investigate the mechanisms of PCF exerting its antiapoptotic effect. The results indicated that PCF, ROS scavenger NAC and NF-kappa B inhibitor MG132 effectively inhibited UVB-induced HaCaT cells apoptosis. PCF (2.84 mM) showed potential ROS scavenging activities in a kinetic process. PCF (1.42-5.69 mM) dose-dependently increased the expressions of Cu, Zn-SOD, CAT and GPx: meanwhile decreased the expressions of p-NF-kappa B/p65 and COX-2 in UVB-induced HaCaT cells. Additionally, pretreatment with NAC significantly declined the generation of ROS and the expression of p-NF-kappa B/p65. We concluded that ROS, NF-kappa B and COX-2 are involved in UVB-induced HaCaT cells apoptosis, PCF exerts its protective effects via scavenging ROS, increasing the expression of antioxidative enzymes and inhibition the activation of NF-kappa B and COX-2. (C) 2009 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Polypeptide From Chlamys Farreri (Pcf) Reactive Oxygen Species (Ros) Nf-kappa b Cox-2 Apoptosis
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.jphotobiol.2009.04.010
关键词[WOS]	NF-KAPPA-B ; ULTRAVIOLET-B ; HUMAN KERATINOCYTES ; SUPEROXIDE-DISMUTASE ; OXIDATIVE STRESS ; MOUSE SKIN ; LIGHT ; RADIATION ; EXPRESSION ; CATALASE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
项目资助者	National Natural Science Foundation of China(30471458) ; Natural Science Foundation of Shandong Province, China(Z2007c09)
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000268406700004
出版者	ELSEVIER SCIENCE SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10438
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Wang, ChunBo
作者单位	1.Qingdao Univ, Coll Med, Qingdao 266071, Peoples R China 2.Laiyang Hlth Sch, Dept Pharmacol, Yantai, Peoples R China 3.E Tennessee State Univ, Dept Biol Sci, Johnson City, TN 37614 USA 4.Chinese Acad Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, XiaoJin,Shi, ShaoTing,Ye, JunLi,et al. Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappa B/COX-2 activation and apoptosis in HaCaT cells[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2009,96(2):109-116.
APA	Liu, XiaoJin,Shi, ShaoTing,Ye, JunLi,Liu, LeQian,Sun, Mi,&Wang, ChunBo.(2009).Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappa B/COX-2 activation and apoptosis in HaCaT cells.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,96(2),109-116.
MLA	Liu, XiaoJin,et al."Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappa B/COX-2 activation and apoptosis in HaCaT cells".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 96.2(2009):109-116.