Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)

doi:10.1016/j.theriogenology.2014.11.004

QIBEBT-IR

	Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)
	Liu, Q. H.1,2; Ma, D. Y.1; Xu, S. H.1; Xiao, Z. Z.1; Xiao, Y. S.1; Song, Z. C.3; Li, J.1,2
	2015-03-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	83 期号:4 页码:703-710
摘要	The present study established an efficient technology for summer flounder (Paralichthys dentatus) sperm cryopreservation, and successfully applied the cryopreserved sperm into interspecific hybridization with olive flounder (P olivaceus). The best motility of postthaw sperm (78.00 +/- 4.70% and 76.60 +/- 7.90%), fertilization rates (95.70 +/- 3.60% and 79.40 +/- 5.20%), and hatching rates (93.10 +/- 4.00% and 77.20 +/- 2.90%) were achieved when using propylene glycol or DMSO as cryoprotectant. Furthermore, we have successfully improved the cryopreservation method from using 2-mL cryotube to 5-mL cryotube, and the dilution ratio has been increased to 4:1. By this method, the cryopreservation efficiency has been improved to 30 times of that with routine method. Computer-assisted sperm motion analysis showed the freezing thawing process decreased the sperm speed but did not significantly change the sperm movement pattern, and the progressive linear motion still was the dominant movement pattern. The ultrastructural analysis showed 50% to 60% of the spermatozoa had normal morphology, 20% to 30% were slightly damaged, such as swelling or rupture of head, midpiece, and tail region, and 10% to 20% were severely damaged. In the artificial hybridization experiment, high fertilization rates and hatching rates were achieved when using 15% DMSO (95.7 +/- 3.6% and 79.4 +/- 5.2%, respectively) and 15% propylene glycol (93.1 +/- 4.0% and 77.2 +/- 2.9%, respectively), with no significant difference in comparison with control (94.7 +/- 2.6% and 72.5 +/- 6.5%, respectively). In addition, we found the embryos and larvae from postthaw sperm of P dentatus developed and grew normally. The results of the present study further validated the safety of the cryopreserved sperm in breeding production by assessing the fertilization capacity, embryo development, and larval growth. (C) 2015 Elsevier Inc. All rights reserved.
文章类型	Article
关键词	Summer Flounder Sperm Cryopreservation Interspecific Hybridization Olive Flounder
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.theriogenology.2014.11.004
关键词[WOS]	SEABREAM PAGRUS-MAJOR ; LARGE-SCALE FERTILIZATION ; RED SEABREAM ; SPARUS-AURATA ; SPERMATOZOA ; GENERATIONS ; 1ST ; L.
收录类别	SCI
语种	英语
WOS研究方向	Reproductive Biology ; Veterinary Sciences
项目资助者	earmarked fund for Modern Agro-industry Technology Research System(nycytx-50) ; National Natural Science Foundation(31072212 ; Science Technology Program of Shandong Province(2011GHY11530) ; Innovation Key Program of the Chinese Academy of Sciences(KSCX2-EW-B-3) ; Key Program of the Chinese Academy of Sciences(KSCX2-EW-B-14) ; Chinese Academy of science and technology service network planning(KFJ-EW-STS-060) ; Fundamental Research Project of Technology Program of Qingdao, China(12-1-4-8(6)-jch ; CAS Scientific and Technological Innovation Program for Cross and Cooperative Team ; 41076100) ; 12-1-4-8-(7)-jch ; 12-4-1-51-hy)
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:000349512800032
出版者	ELSEVIER SCIENCE INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10428
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li, J.
作者单位	1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China 3.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China
推荐引用方式 GB/T 7714	Liu, Q. H.,Ma, D. Y.,Xu, S. H.,et al. Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)[J]. THERIOGENOLOGY,2015,83(4):703-710.
APA	Liu, Q. H..,Ma, D. Y..,Xu, S. H..,Xiao, Z. Z..,Xiao, Y. S..,...&Li, J..(2015).Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus).THERIOGENOLOGY,83(4),703-710.
MLA	Liu, Q. H.,et al."Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)".THERIOGENOLOGY 83.4(2015):703-710.