QIBEBT-IR研究单元&专题: 资源植物与环境工程研究组
http://ir.qibebt.ac.cn:80/handle/0/140
2024-03-28T20:55:56Z
2024-03-28T20:55:56Z
亚麻荠种皮粘液质多糖特性及其相关调控基因的分析
鲁明闽
http://ir.qibebt.ac.cn:80/handle/337004/13653
2019-12-31T10:57:01Z
2019-12-31T10:57:01Z
题名: 亚麻荠种皮粘液质多糖特性及其相关调控基因的分析
作者: 鲁明闽
2019-12-31T10:57:01Z
一种降低木质素含量的代谢途径改造方法及应用
周功克
祁广
柴国华
孔英珍
王殿
唐贤丰
胡瑞波
http://ir.qibebt.ac.cn:80/handle/337004/13571
2019-12-31T10:39:42Z
2019-12-31T10:39:39Z
题名: 一种降低木质素含量的代谢途径改造方法及应用
作者: 周功克; 祁广; 柴国华; 孔英珍; 王殿; 唐贤丰; 胡瑞波
2019-12-31T10:39:39Z
一种易降解植物细胞壁的分子设计及应用
周功克
祁广
柴国华
孔英珍
王殿
唐贤丰
付春祥
贺郭
http://ir.qibebt.ac.cn:80/handle/337004/13570
2019-12-31T10:39:40Z
2019-12-31T10:39:37Z
题名: 一种易降解植物细胞壁的分子设计及应用
作者: 周功克; 祁广; 柴国华; 孔英珍; 王殿; 唐贤丰; 付春祥; 贺郭
2019-12-31T10:39:37Z
拟南芥CCCH锌指蛋白C3H14/15调控植物抗病的分子机理
王殿
http://ir.qibebt.ac.cn:80/handle/337004/13478
2021-07-31T02:44:16Z
2018-12-29T09:33:25Z
题名: 拟南芥CCCH锌指蛋白C3H14/15调控植物抗病的分子机理
作者: 王殿
2018-12-29T09:33:25Z
Insights into the structural and physicochemical properties of small granular starches from two hydrophyte duckweeds, Spirodela oligorrhiza and Lemna minor
Chen, Lei
Yu, Changjiang
Ma, Yubin
Xu, Hua
Wang, Shumin
Wang, Yu
Liu, Xingxun
Zhou, Gongke
http://ir.qibebt.ac.cn:80/handle/337004/10454
2019-12-31T08:33:26Z
2018-09-06T06:57:20Z
题名: Insights into the structural and physicochemical properties of small granular starches from two hydrophyte duckweeds, Spirodela oligorrhiza and Lemna minor
作者: Chen, Lei; Yu, Changjiang; Ma, Yubin; Xu, Hua; Wang, Shumin; Wang, Yu; Liu, Xingxun; Zhou, Gongke
摘要: The structure and physicochemical properties of starches from two hydrophyte duckweeds, Spirodela oligorrhiza and Lemna minor, were investigated and compared in this study. The amylose content and average size of starches were determined to be 20.85%, 4.70 mu m and 27.77%, 6.17 mu m for Spirodela oligorrhiza and Lemna minor, respectively. The average chain length of two duckweed starches was measured to be around DP 28. The chain length distribution was observed to be greatly different from other reported starches for the high proportion of long chains (DP >= 37) over 50%. Wide-angle X-ray diffraction profiles of the two starch samples displayed typical B-type diffraction pattern. The gelatinization enthalpy-changes (Delta H-gel) of two starch samples was about 10.40 J/g for two duckweed starches. The present results suggested the potential utilization of small granular starches from duckweed in functional foods and dietary supplement products. (C) 2016 Elsevier Ltd. All rights reserved.
2018-09-06T06:57:20Z
HDG2转录因子调控拟南芥种皮粘液质结构的机制研究
裴胜强
http://ir.qibebt.ac.cn:80/handle/337004/9978
2018-08-27T22:57:29Z
2018-01-08T10:28:55Z
题名: HDG2转录因子调控拟南芥种皮粘液质结构的机制研究
作者: 裴胜强
摘要: As the most abundant renewable resource on the earth, plant cell wall has numerous applications in various aspects of human living and industry servering as the main source of energy, fiber, building materials and papermaking materials. Thus, in-depth analysis of the composition, structure and biosynthesis mechanisms of plant cell wall, and optimization of the composition, structure and connections between different components by genetic engineering to improve the saccharification efficiency , is hot topics in the research of plant cell wall.
Arabidopsis thaliana seed coat cells synthesize and secrete large amounts of mucilage ploysaccarides (mainly composed of rhamnogalactoside I, RG I) to the extracellular at specific satges during the seed coat differentiation.Besides pectic RG I, mucilage also consists a small amount of cellulose and hemicellulose (e.g. heteroxylansand glactoglucomannan) components, thus mucilage represnet a specialized cell wall. Seed coat mucilage is not necessary for the germination or growth of plants, in addition, seed coat mucilage can be easily extracted and the defect phenotype can be readily identified, so it can be utilized as an ideal model system for the study of biosynthesis, modification and regulation of cell wall polysaccharides.
Previous study identified a transcription factor gene, HOMODOMAIN GLABROUS 2 (HDG2) . HDG2 belongs to homeobox subfamily HD-Zip IV. This study mainly focused on the mechanism of HDG2 in the regulation of seed coat mucilage structure.The main results are as follows: 1) Real-time quantitative PCR (qRT-PCR), in situ hybridization and promoter GUS activity analysis showed that HDG2 was specifically expressed in the seed coat, and its peak was observed at 10 days after pollination (10 DPA). 2) The transcriptional activation activity of HDG2 and its transcriptional activation domain was analyzed in yeast cell. The truncation test was performed according to the conserved domain of HDG2 protein (HD, LZ, START and SAD). The results indicated that HDG2 had transcriptional activation activity, and its transcriptional activation is located in the leucine zipper (LZ) domain. 3) Ruthenium red staining was carried out for T-DNA insertion mutants of HDG2(hdg2-2 and hdg2-3). The results showed that seed coat mucilage of hdg2 can be normally released, and no significant changes in total amount was observed. However, the mucilage layers of hdg2 was easily shaked off, leaving thinner adherent inner mucilage than that of the wild type (WT). 4) The hdg2 and WT seeds were dissected from 4 DPA-13 DPA and observed by resin embedding and slicing, the results showed that epidermal cells of hdg2-3 seed were normally developed and the secretion and the amount of mucilage displayed no significant differences compared to the WT. 5) The content, composition and structure of hdg2-3 mucilage was analyzed, and it was found that the total amount of hdg2-3 mucilage had no significant differences with that of the WT. The composition of monosaccharide showed that the contents of monosaccharides,which affect the mucilage structure (e.g. xylose, mannose) had no significant differences with the WT. 6) In situ immunohistochemical analysis showed that the crystalline cellulose content was significantly reduced in hdg2-3 compare with the WT, which was confirmed by the quantification of crystalline cellulose contents. Thus it can be conclueded that the decrease of crystalline cellulose led to the mucilage defect in hdg2-3. 7) To further confirm if HDG2 is involved in the modulation of crystalline cellulose, qRT-PCR analysis of hdg2 and WT seeds at different stages was performed. The results showed that CESA5, a key gene affecting cellulose synthesis in seed coat mucilage, was significantly decreased in hdg2-3. EMSA and yeast one hybridization analysis confirmed that HDG2 protein could bind to the L1-box motif located in CESA5 promoter. In addition, protoplast transcriptional activation assay showed that HDG2 could activate the expression of CESA5.
In summary, the molecular mechanisms of HDG2 transcription factor in the regulation of mucilage structure were characterzide in this study. A series of physiological and biochemical evidence suggested that HDG2 regulates the synthesis or assembly of cellulose by direct activation of CESA5 to maintain the normal structure of the seed coat mucilage. In contrast, HDG2 mutations inhibit the expression of CESA5, the synthesis or assembly of crystalline cellulose is blocked and finally showing a defective phenotype of the seed coat mucilage. The results obtained will help to deepen our understanding of the molecular mechanisms and regulation networks underlying cellulose synthesis and assembly, which lay a theoretical foundation for the future custom-design of the cell wall by genetic engineering means.
摘要: 中文
2018-01-08T10:28:55Z
Inoculation of AM Fungi: An Effective Tool to Reduce Cd Accumulationin Peanut Kernel. International
Mingliang Li, Wenjing Cui, Yamei Zhuang, Lei Zhang
http://ir.qibebt.ac.cn:80/handle/337004/9833
2018-08-27T23:02:08Z
2018-01-04T19:52:03Z
题名: Inoculation of AM Fungi: An Effective Tool to Reduce Cd Accumulationin Peanut Kernel. International
作者: Mingliang Li, Wenjing Cui, Yamei Zhuang, Lei Zhang
摘要: China is an important global peanut producer and exporter. Nevertheless, excessive Cd in peanut kernels has become an
important constraint of peanut exportation. Concerns over the heavy metal contamination in food crops have prompted
scientists to find ways to solve these problems, including applications of microbiology. In the present research, a greenhouse
pot culture experiment was conducted to investigate the effect of arbuscular mycorrhizal fungi (AMF) inoculation on cadmium
(Cd) uptake and translocation in peanut plants at different Cd levels. The peanut seeds were sown in pots, where culture
substrate was previously mixed with a given amount of Glomus intraradices and 0, 2 and 10 mg kg -1 Cd. In the control plot,
G. intraradices was inactivated before mixed into culture substrate. Symbiotic relationships were successfully established
between AMF and peanut root at all Cd levels with an average colonization rate of 65.0%. Compared with control plants,
AMF inoculation significantly improved phosphorus nutrition supply to peanut plants, increased chlorophyll by 7.5%,
photosynthesis by 11.8%, transpiration by 13.9% and root dry weight by 27.0%. In AMF inoculated peanut plants, the
concentration and accumulation of Cd were 45.9 and 87.4% higher, respectively, in root system but 31.1 and 31.8% lower,
respectively in the aboveground part than in the control plants. At the Cd level of 10 mg kg -1 , the translocation rate of Cd in
AMF inoculated peanut plants was 51.8% lower than in AMF non-inoculated plants. In summary, AMF inoculation could
improve peanut plant growth, result in Cd immobilisation in the peanut root system and inhibit Cd translocation in peanut
plants. In conclusion, AMF inoculation may be an effective way to reduce Cd accumulation in peanut kernels. © 2017 Friends
Science Publishers
2018-01-04T19:52:03Z
甘蓝型油菜作图群体亲本基因组及性状QTL密集区变异特点
程明星,童超波,程晓晖,刘越英,刘立江,柴国华,黄军艳*,刘胜毅.
http://ir.qibebt.ac.cn:80/handle/337004/9834
2018-08-27T23:02:08Z
2018-01-04T19:52:03Z
题名: 甘蓝型油菜作图群体亲本基因组及性状QTL密集区变异特点
作者: 程明星,童超波,程晓晖,刘越英,刘立江,柴国华,黄军艳*,刘胜毅.
摘要: 为了解作图群体亲本间基因组和密集QTL区的遗传变异,促进QTL定位与克隆,本研究对一个甘蓝型油菜(Brassica napus)重组自交系(recombinant lnbred lines,RIL)群体双亲M083和888-5(二者株高、分枝数、开花时间、菌核病抗性等性状差异显著)的基因组进行深度测序和分析。研究结果表明:在M083和888-5中分别检测到SNP个数为1 941 397和2 046 009,In Del个数为410 961和428 572,结构变异个数为90 384和88 456,拷贝数变异个数为46 655和46 008。对包含开花时间、菌核病抗性、株高等多个性状主效QTL密集的A02染色体进行分析发现,在包括上述性状QTL密集的6.4~6.9Mb的0~8.5Mb基因组区间内,两品系SNPs和In Dels变异数量/密度总体呈相反的趋势;片段丢失也有热点区,888-5和M083均在两个共同区域高频发生,其中一个为QTL密集区;倒位在染色体上的分布虽然也是不均匀的,但两品种间发生区域不同,且与QTL无关。本文关于基因组变异(SNPs、In Dels、除倒位外的结构变异)热点区和QTL密集区重复的结论对性状控制位点精细定位、基因克隆及育种亲本选择有一定的指导意义
2018-01-04T19:52:03Z
DGE-seq analysis of MUR3-related Arabidopsis mutants providesinsightinto how dysfunctional xyloglucan affects cell elongation
Zongchang Xu , Meng Wang, Dachuan Shi , Gongke Zhou, Tiantian Niu,
Michael G. Hahn, Malcolm A. O’Neill , Yingzhen Kong
http://ir.qibebt.ac.cn:80/handle/337004/9832
2018-08-27T23:02:19Z
2018-01-04T19:52:02Z
题名: DGE-seq analysis of MUR3-related Arabidopsis mutants providesinsightinto how dysfunctional xyloglucan affects cell elongation
作者: Zongchang Xu , Meng Wang, Dachuan Shi , Gongke Zhou, Tiantian Niu,
Michael G. Hahn, Malcolm A. O’Neill , Yingzhen Kong
摘要: Our previous study of the Arabidopsis mur3-3 mutant and mutant plants in which the mur3-3 phenotypes are suppressed (xxt2mur3-3, xxt5mur3-3, xxt1xxt2mur3-3 and 35Spro:XLT2:mur3-3) showed that hypocotyl cell elongation is decreased in plants that synthesize galactose-deficient xyloglucan. To obtain genome-wide insight into the transcriptome changes and regulatory networks that may be involved in this decreased elongation, we performed digital gene expression analyses of the etiolated hypocotyls of wild type (WT), mur3-3 and the four suppressor lines. Numerous differentially expressed genes (DEGs) were detected in comparisons between WT and mur3-3 (1423), xxt2mur3-3 and mur3-3 (675), xxt5mur3-3 and mur3-3 (1272), xxt1xxt2mur3-3 and mur3-3 (1197) and 35Spro:XLT2:mur3-3 vs mur3-3 (121). 550 overlapped DEGs were detected among WT vs mur3-3, xxt2mur3-3 vs mur3-3, xxt5mur3-3 vs mur3-3, and xxt1xxt2mur3-3 vs mur3-3 comparisons. These DEGs include 46 cell wall-related genes, 24 transcription factors, 6 hormone-related genes, 9 protein kinase genes and 9 aquaporin genes. The expression of all of the 550 overlapped genes is restored to near wild-type levels in the four mur3-3 suppressor lines. qRT-PCR of fifteen of these 550 genes showed that their expression levels are consistent with the digital gene expression data. Overexpression of some of these genes (XTH4, XTH30, PME3, EXPA11, MYB88, ROT3, AT5G37790, WAG2 and TIP2;3) that are down-regulated in mur3-3 partially rescued the short hypocotyl phenotype but not the aerial phenotype of mur3-3, indicating that different mechanisms exist between hypocotyl cell elongation and leaf cell elongation. (C) 2017 Elsevier B.V. All rights reserved
2018-01-04T19:52:02Z
甘蓝型油菜 CCCH 基因 BnaA07g26050D的克隆及表达分析
王殿,庄亚妹,徐华,祁广,周功克,柴国华
http://ir.qibebt.ac.cn:80/handle/337004/9831
2018-08-27T23:02:20Z
2018-01-04T19:52:01Z
题名: 甘蓝型油菜 CCCH 基因 BnaA07g26050D的克隆及表达分析
作者: 王殿,庄亚妹,徐华,祁广,周功克,柴国华
摘要: 油菜是我国重要的油料作物ꎬ它的产量受到各种逆境胁迫的影响.我们之前的研究表明ꎬ拟南芥 CCCH 锌指蛋白
AtC3H14 控制植株生长发育的诸多过程.在本研究中ꎬ我们证实甘蓝型油菜 CCCH 基因 BnaA07g26050D(AtC3H14 的同源基因)响
应盐胁迫和干旱胁迫.BnaA07g26050D 蛋白 C 端包含两个串联的 CX 8 CX 5 CX 3 H 结构域ꎬ在酵母中具有转录激活活性ꎬ类似于
AtC3H14.将 BnaA07g26050D 融合 GFP 转化拟南芥原生质体ꎬ 发现其主要定位于细胞质和 P - body 中. qRT - PCR 检 测
BnaA07g26050D 基因的组织表达模式ꎬ结果表明该基因主要在上部茎、根和花中大量表达ꎬ而在其他组织中表达较低.此外ꎬqRT-
PCR 证实 BnaA07g26050D 基因在盐胁迫和干旱胁迫下表达量都明显上调ꎬ证明该基因可能参与干旱和盐胁迫.我们的研究为利用
基因工程技术培育耐盐、旱油菜新品种提供了基因源.
2018-01-04T19:52:01Z