QIBEBT-IR研究单元&专题: 分子微生物工程研究组http://ir.qibebt.ac.cn:80/handle/0/1392024-03-29T14:51:31Z2024-03-29T14:51:31Z红法夫酵母合成虾青素代谢工程研究张宁http://ir.qibebt.ac.cn:80/handle/337004/136632019-12-31T10:57:07Z2019-12-31T10:57:07Z题名: 红法夫酵母合成虾青素代谢工程研究
作者: 张宁2019-12-31T10:57:07Z解脂耶罗维亚酵母中磷脂酶A2基因功能的研究李家欣http://ir.qibebt.ac.cn:80/handle/337004/136562019-12-31T10:57:03Z2019-12-31T10:57:03Z题名: 解脂耶罗维亚酵母中磷脂酶A2基因功能的研究
作者: 李家欣2019-12-31T10:57:03Z乙酰海藻酸钠酯酶与海藻酸钠裂解酶协同降解铜绿假单胞杆菌生物膜研究谭芳呈http://ir.qibebt.ac.cn:80/handle/337004/136502019-12-31T10:56:59Z2019-12-31T10:56:59Z题名: 乙酰海藻酸钠酯酶与海藻酸钠裂解酶协同降解铜绿假单胞杆菌生物膜研究
作者: 谭芳呈2019-12-31T10:56:59Z一种耐热木聚糖酶及其应用李福利孟冬冬吕明张坤迪http://ir.qibebt.ac.cn:80/handle/337004/135592019-12-31T10:39:24Z2019-12-31T10:39:22Z题名: 一种耐热木聚糖酶及其应用
作者: 李福利; 孟冬冬; 吕明; 张坤迪2019-12-31T10:39:22ZThermophilic MicroorganismsFu-Li Lihttp://ir.qibebt.ac.cn:80/handle/337004/108472018-12-20T22:03:10Z2018-12-20T08:20:00Z题名: Thermophilic Microorganisms
作者: Fu-Li Li2018-12-20T08:20:00ZCharacterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes aferFan, YongYuan, ChengJin, YiHu, Guang-RongLi, Fu-Lihttp://ir.qibebt.ac.cn:80/handle/337004/107202018-09-06T06:59:13Z2018-09-06T06:59:12Z题名: Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer
作者: Fan, Yong; Yuan, Cheng; Jin, Yi; Hu, Guang-Rong; Li, Fu-Li
摘要: Nervonic acid (C24:1, Delta 15), a monounsaturated long chain fatty acid, is a major component of mammalian neural tissue. Lack of nervonic acid in human usually leads to neurological disorder. Recently, production of nervonic acid from plant for pharmaceutical, nutraceutical, and functional food received increasing attention. We have previously isolated a microalgal strain Mychonastes afer HSO-3-1, which can accumulate approximately 6% of nervonic acid and <0.5% of erucic acid in triacylglycerol. In this study, the full length of 3-ketoacyl-coA synthase gene MaKCS, encoding the first component of fatty acid elongation complex, was cloned and expressed in Saccharomyces cerevisiae BY4741 under the control of GAL1 promoter. S. cerevisiae harboring pYC230-Gal-MaKCS resulted in the production of 1.5% nervonic acid in total lipid. The expression of KCS gene in M. afer was found to be induced under stress conditions such as nitrogen deficiency and high light, which was revealed by real-time PCR analysis. MaKCS was demonstrated to be able to extend very long chain monounsaturated fatty acids including nervonic acid, which provides insights into how nervonic acid is synthesized in microalgae.2018-09-06T06:59:12ZSubstitution of one calcium-binding amino acid strengthens substrate binding in a thermophilic alginate lyaseWang, BingJi, Shi-QiMa, Xiao-QingLu, MingWang, Lu-ShanLi, Fu-Lihttp://ir.qibebt.ac.cn:80/handle/337004/106362018-09-06T06:58:37Z2018-09-06T06:58:37Z题名: Substitution of one calcium-binding amino acid strengthens substrate binding in a thermophilic alginate lyase
作者: Wang, Bing; Ji, Shi-Qi; Ma, Xiao-Qing; Lu, Ming; Wang, Lu-Shan; Li, Fu-Li
摘要: Ligand binding is sensitive to temperatures since noncovalent bonds between the binding site and ligand could be broken by heat. How metal ion-binding amino acids in alginate lyase evolve to achieve tight substrate binding in a hostile environment remains unknown. An endolytic alginate lyase AlgAT0 specifically cleaved the M-G glycosidic bond and released disaccharides as the main end product. Four conserved calcium-binding sites were predicted and the supplement of Ca2+ led to enhanced substrate binding and protein stability. Among the four conserved calcium-binding sites, one substitution of aspartate for glutamate in AlgAT0 was proved to stimulate Ca2+ affinity. This study suggested that substrate affinity of polysaccharide lyases could be improved by tight binding to Ca(2+)via one amino acid substitution.2018-09-06T06:58:37ZClostridium cellulosi多模块内切纤维素酶CcCel9A持续性机制研究张坤迪http://ir.qibebt.ac.cn:80/handle/337004/99602018-08-27T22:57:56Z2018-01-08T10:00:58Z题名: Clostridium cellulosi多模块内切纤维素酶CcCel9A持续性机制研究
作者: 张坤迪2018-01-08T10:00:58Z热解纤维素菌半纤维素降解机制研究Md. Abu Saleh,http://ir.qibebt.ac.cn:80/handle/337004/99482018-08-24T00:58:50Z2018-01-08T10:00:47Z题名: 热解纤维素菌半纤维素降解机制研究
作者: Md. Abu Saleh,2018-01-08T10:00:47Z微藻育种研究进展.范勇, 胡光荣, 王丽娟, 李福利*.http://ir.qibebt.ac.cn:80/handle/337004/98832018-08-27T23:01:24Z2018-01-05T14:15:01Z题名: 微藻育种研究进展.
作者: 范勇, 胡光荣, 王丽娟, 李福利*.2018-01-05T14:15:01Z